利用高效薄层层析法,以罗布麻浸膏中的槲皮素为标准,测定罗布麻烟中罗布麻浸膏的含量。以甲苯:甲酸:乙酸乙酯:正己烷(2:0.2:1.5:0.2)作展开剂。可使得药烟中槲皮素与其它成分很好分离。分离后,用薄层扫描仪于波长410nm,参比波长750 nm扫描定量。本法需样品少,快速简便、灵敏准确。可适用于药烟生产厂家化验室检测使用。 The content of Apocynum extract in A. venetum was determined by high performance thin layer chromatography and quercetin in Apocynum extract. Toluene: formic acid:ethyl acetate:n-hexane (2:0.2:1.5:0.2) was used as a developing solvent. It can make the quercetin in the tobacco smoke well separated from other ingredients. After separation, quantification was performed using a thin layer scanner at a wavelength of 410 nm and a reference wavelength of 750 nm. This method requires less samples, quick and easy, sensitive and accurate. Applicable to drug manufacturers testing laboratory testing and use.