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目的对一起食物中毒检出的3种血清型副溶血性弧菌(Vibrio parahaemolyticus,VP),采用脉冲场凝胶电泳(PFGE)进行同源性分析,以明确其传染源。方法参照WS 271-2007等标准,进行副溶血性弧菌分离、生化和血清学鉴定、KP试验、药敏试验、毒力基因检测、PFGE分子分型,利用BioNumerics软件对PFGE分型图谱进行聚类分析。结果 17份样品均检出副溶血性弧菌,血清型可分3个型别,以O3∶K6为优势血清型,占70.59%,O2∶K3和O2∶K28两血清型所占比例不一。PFGE分子分型,按100%相似度可分6个基因型别,XC12001型为优势型别,占58.82%,XC12002～XC12006型所占比例不一,聚类分析显示XC12001和XC12002型、XC12004和XC12005型相似度较高。17株副溶血性弧菌药敏结果基本一致,对氨苄西林和阿莫西林耐药率高达100%。10株O3∶K6型副溶血性弧菌只携带tdh毒力基因,其余菌株tdh、trh1、trh2毒力基因全部阴性。结论此起食物中毒是以O3∶K6血清型为主的不同克隆群副溶血性弧菌混合感染所致,PFGE可有效应用于食物中毒溯源分析及分子流行病学研究。
Objective To analyze the homology of three serotype Vibrio parahaemolyticus (VP) isolated from food poisoning by using pulsed-field gel electrophoresis (PFGE) to identify the source of infection. Methods According to the standard of WS 271-2007, Vibrio parahaemolyticus, biochemical and serological identification, KP test, drug susceptibility test, virulence gene test and PFGE molecular typing were carried out. Bioinformatics software was used to collect PFGE genotyping map Class analysis Results Vibrio parahaemolyticus was detected in all the 17 samples. The serotypes were divided into 3 types. O3: K6 was the predominant serotype, accounting for 70.59%. The serogroups of O2: K3 and O2: K28 were different in proportion . PFGE molecular typing, according to 100% similarity can be divided into 6 genotypes, XC12001 type is the dominant type, accounting for 58.82%, XC12002 ~ XC12006 type accounting for different proportions, cluster analysis showed XC12001 and XC12002 type, XC12004 and XC12005 type of similarity is higher. 17 strains of Vibrio parahaemolyticus drug susceptibility results are basically the same, the resistance rate of ampicillin and amoxicillin up to 100%. Ten strains of O3: K6 Vibrio parahaemolyticus carried only the tdh virulence genes, while the rest strains tdh, trh1 and trh2 virulence genes were all negative. Conclusion This food poisoning is caused by the mixed infection of Vibrio parahaemolyticus with different clonal groups based on O3: K6 serotype. PFGE can be effectively applied in the traceability analysis and molecular epidemiology of food poisoning.