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实验旨在对含载金属离子沸石粉抗菌纸的抗菌性能进行检测。采用液体连续稀释法和96孔板检测法检测载金属离子沸石粉对细菌(大肠埃希菌E.coli、金黄色葡萄球菌S.aureus)和真菌(白色念珠菌M.albican、黑根霉R.nigricans、黑曲霉A.niger)的最小抗菌浓度(MIC),以及平板菌落计数法对抗菌纸的抗菌率进行检测(抗菌率计算公式为:X 5=(A-B)/A×100%,其中X 5为抗菌率,A为对照平板菌落,B为样品平板菌落)。载金属离子沸石粉对大肠埃希菌、金黄色葡萄球菌、白色念珠菌的MIC分别达到1×10~(-7)g/m L、1×10~(-5)g/m L、1×10~(-6)g/m L,对根霉和曲霉也具有一定的抑制效果。抗菌纸对大肠埃希菌、金黄色葡萄球菌、白色念珠菌的抗菌率均在48%以上,完全符合《一次性卫生用品卫生标准》中所规定的抗菌率在26%以上的标准。
The experiment aims to test the antibacterial property of antibacterial paper containing metal ion zeolite powder. Bacterial (Escherichia coli, S. aureus) and fungi (M.albican, Rhizopus oryzae R) were tested by liquid serial dilution and 96-well plate assay. .nigricans, A. niger) and the antibacterial rate of the antibacterial paper by the colony counting method (the antibacterial rate was calculated as: X 5 = (AB) / A × 100%, in which X 5 is the antibacterial rate, A is the control plate colonies, B is the sample plate colonies). The MIC of zeolite loaded with metal ion to Escherichia coli, Staphylococcus aureus and Candida albicans respectively reached 1 × 10 -7 g / m L, 1 × 10 -5 g / m L, 1 × 10 ~ (-6) g / m L, also has certain inhibitory effect on Rhizopus and Aspergillus. Antibacterial paper antibacterial rate of Escherichia coli, Staphylococcus aureus, Candida albicans were more than 48% antibacterial, in full compliance with “hygiene standards for disposable hygiene products” in the antimicrobial rate of 26% or more.