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The effect of carboxymethytl pachymaram ( CMP ) on the function of dendritic cells(DCs) derived from spleens of hepatitis B virus transgenic mice are studied in vitro. The phenotypes of DCs are tested by flow cytometry (FCM), cytokines measured by ELISA. The expression of DCs' phenotypes in HBV transgenic mice are low (CD80~+CD11c~+:59.12±11.53 vs 9.60±4.53, p<0.01; CD80+ MHC-Ⅱ~+: 44.86±12.31 vs 9.80±5.72, p<0.01, normal mice vs HBV transgenic mice), the ability of DCs stimulating T lymphocytes proliferation decreases (0.37±0.11 vs 0.20±0.11, p<0.05, normal mice vs HBV transgenic mice), levels of IL-12 and IFN-γ decrease whereas the level of IL-10 increases; CMP can enhance DCs' ability of stimulating T lymphocytes proliferation, facilitate the secretion of IL-12 and IFN-γ, inhibit the secretion of IL-10, thus up regulates DCs function. The results show a good prospective use of CMP on the treatment of chronic hepatitis B.
The effect of carboxymethytl pachymaram (CMP) on the function of dendritic cells (DCs) derived from spleens of hepatitis B virus transgenic mice were studied in vitro. The phenotypes of DCs are tested by flow cytometry (FCM), cytokines measured by ELISA. expression of DCs' phenotypes in HBV transgenic mice are low (CD80 ~ + CD11c ~ +: 59.12 ± 11.53 vs 9.60 ± 4.53, p <0.01; CD80 + MHC- Ⅱ ~ +: 44.86 ± 12.31 vs 9.80 ± 5.72, normal mice vs HBV transgenic mice), the ability of DCs stimulating T lymphocytes decrease decreases (0.37 ± 0.11 vs 0.20 ± 0.11, p <0.05, normal mice vs HBV transgenic mice), levels of IL-12 and IFN- levels of IL-10 increases; CMP can enhance DCs' ability of stimulating T lymphocytes proliferation, facilitate the secretion of IL-12 and IFN-γ, inhibit the secretion of IL-10, thus up regulates DCs function. The results show a good prospective use of CMP on the treatment of chronic hepatitis B