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目的探讨肿瘤坏死因子相关凋亡诱导配体(TRAIL)联合水飞蓟素(Sili)对人肝癌Huh7细胞凋亡的影响。方法 CCK8法检测细胞增殖抑制;分光光度法检测细胞caspase-3、caspase-8、caspase-9相对活性;Western blot检测细胞表面死亡受体4(DR4)、死亡受体5(DR5)的表达。结果 Sili与TRAIL联合作用Huh7细胞后,联合用药组与对照组及单独用药组相比,Huh7细胞的增殖率明显下降;caspase-3、caspase-8、caspase-9的相对活性明显提高。Sili作用Huh7细胞后能明显提高DR4、DR5蛋白表达。结论 Sili可通过上调死亡受体DR4、DR5表达而促进TRAIL诱导的Huh7细胞凋亡,为肝细胞癌的临床治疗提供了新的思路和方法。
Objective To investigate the effects of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) combined with silymarin (Sili) on the apoptosis of human hepatoma Huh7 cells. Methods CCK8 was used to detect the cell proliferation inhibition. The relative activities of caspase-3, caspase-8 and caspase-9 were detected by spectrophotometry. The expressions of DR4 and DR5 were detected by Western blot. Results After Sili and TRAIL combined with Huh7 cells, the proliferation rate of Huh7 cells was significantly decreased compared with the control group and the drug alone group. The relative activities of caspase-3, caspase-8 and caspase-9 were significantly increased. Sili effect on Huh7 cells can significantly improve DR4, DR5 protein expression. Conclusion Sili can promote the apoptosis of Huh7 cells induced by TRAIL by up-regulating DR4 and DR5 expression of death receptors, and provide new ideas and methods for the clinical treatment of hepatocellular carcinoma.