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目的研究天目地黄Rehmannia chingii全草的化学成分。方法通过正相硅胶、Sephadex LH-20以及反相HPLC柱等色谱方法对天目地黄的化学成分进行分离纯化,应用谱学技术鉴定化合物的结构。结果从天目地黄水提取物中分离得到17个化合物,分别鉴定为8-甲基-9氢环戊烷并吡喃-1,3,6,8-四醇(1)、3,4-二羟基苯乙醇(2)、3-甲氧基-4-羟基苯乙醇(3)、苯乙醇-8-O-β-D-葡萄糖苷(4)、(3,4-二羟基苯乙醇)-鼠李糖-(1→3)-葡萄糖苷(5)、(苯乙醇)-木糖-(1→6)-葡萄糖苷(6)、去乙酰地黄苷(7)、毛蕊花糖苷(8)、异毛蕊花糖苷(9)、地黄苷(10)、异地黄苷(11)、焦地黄苯乙醇苷C(12)、焦地黄苯乙醇苷A1(13)、焦地黄苯乙醇苷B1(14)、3,4-dihydroxy-β-phenethyl-O-α-L-rhamnopyranosyl-(1→3)-O-β-D-galacopyranosyl-(1→6)-4-O-caffeoyl-β-D-glucopyranoside(15)、肉苁蓉苷F(16)、异肉苁蓉苷F(17)。结论共鉴定17个化合物,包括1个环烯醚萜(1),16个苯乙醇及其苷(2~17);化合物1为新化合物,命名为天目地黄素;化合物16、17为首次从该属植物中分离得到。经体外活性筛选发现化合物9、12、14~16有醛糖还原酶抑制活性。
Objective To study the chemical constituents of Rehmannia chingii. Methods The chemical constituents of Rehmanniae Radix were isolated and purified by normal phase silica gel, Sephadex LH-20 and reverse phase HPLC. The structures of the compounds were identified by spectroscopic methods. Results Seventeen compounds were isolated from Tianmuhuang extract, and identified as 8-methyl-9-cyclopentanopyran-1,3,6,8-tetrol (1), 3,4- (3), phenethyl alcohol-8-O-β-D-glucoside (4), (3,4-dihydroxyphenethyl alcohol) - (1 → 3) -Glucoside (5), (Phenethyl alcohol) -Xylose- (1 → 6) -Glucoside (6), Deacetylglucoside (7), Verbascoside (8), Isovermolin glycosides (9), recuperan glycoside (10), alloside (11), pyrograd coenzyme C (12), pyrogradone A1 (13) (1 → 3) -O-β-D-galacopyranosyl- (1 → 6) -4-O-caffeoyl-β-D-glucopyranoside (3,4- dihydroxy-β-phenethyl-O-α-L-rhamnopyranosyl- 15), Cistanin F (16), Cistanin F (17). Conclusion A total of 17 compounds were identified, including 1 iridoid (1), 16 phenethyl alcohol and its glycosides (2 ~ 17). Compound 1 was a new compound named as cephalosporin. Compounds 16 and 17 The genus was isolated. Compounds 9,12,14 ~ 16 were found to have aldose reductase inhibitory activity by in vitro activity screening.