论文部分内容阅读
目的:探讨汉族人内皮型一氧化氮合酶(eNOS)基因内含子4可变数目串联重复序列插入/缺失多态性(4b/a)与2型糖尿病(T2DM)合并高尿酸血症的关系。方法:提取100例健康人(A组)、76例T2DM无高尿酸血症患者(B组)和124例T2DM合并高尿酸血症患者(C组)的基因组DNA,用聚合酶链反应-可变数目串联重复序列法测定4b/a多态性。结果:3组eNOS基因内含子4等位基因的分布和频率差异有统计学意义(P<0.05)。T2DM所有患者中4b/b、4b/4a、4a/4a各基因型高尿酸血症的发病率逐渐升高分别为35.4%、58.8%和100%。4b和4a等位基因患者高尿酸血症的发病率分别为36.5%和63.6%,两者比较差异有统计学意义。携带a等位基因的T2DM患者较未携带a等位基因者合并高尿酸血症者的危险性升高(4b/4a的OR为3.42,P<0.05)。结论:eNOS4a等位基因是T2DM患者并发高尿酸血症的独立危险因素,可能为筛查T2DM患者高尿酸血症的高危人群提供侯选基因。
Objective: To investigate the association between intron 4 intron 4 intron 4 (4b / a) polymorphism and type 2 diabetes mellitus (T2DM) in Han nationality with eNOS gene hyperuricemia relationship. Methods: The genomic DNA of 100 healthy subjects (group A), 76 T2DM patients without hyperuricemia (group B) and 124 patients with T2DM complicated with hyperuricemia (group C) were extracted and analyzed by polymerase chain reaction Variable number tandem repeat method for the determination of 4b / a polymorphism. Results: The distribution and frequency of intron 4 alleles of eNOS gene in 3 groups were statistically significant (P <0.05). The incidence of hyperuricemia of all genotypes 4b / b, 4b / 4a and 4a / 4a in T2DM patients were 35.4%, 58.8% and 100% respectively. The incidences of hyperuricemia in patients with alleles 4b and 4a were 36.5% and 63.6%, respectively, with significant differences between the two groups. T2DM patients with a allele had a higher risk of having hyperuricemia than those who did not carry a allele (OR of 4b / 4a was 3.42, P <0.05). Conclusion: The eNOS4a allele is an independent risk factor for hyperuricemia in T2DM patients and may provide candidate genes for screening high-risk population of hyperuricemia in T2DM patients.