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血小板上鉴定出ABH血型决定簇已有多年,但有关该决定簇在血小板膜上定位的资料极少。作者应用多项技术,鉴定出至少有3种不同的血小板糖蛋白(GP)即GP Ⅱ a,Ⅲ a和Ⅰb带有A、B决定簇,其中GP Ⅱ a最为显著。作者应用经血小板吸收和酸洗脱纯化的高效价IgG抗-A、抗-B血清,各种糖蛋白单克隆抗体,以放射免疫沉淀法、免疫印迹术和单克隆抗体固化血小板抗原法(MAIPA),对血小板A、B血型决定簇在血小板糖蛋白上的定位,作了精确的测定。
The ABH blood group determinant has been identified on platelets for many years, but there is very little information on the location of this determinant on the platelet membrane. The authors applied a number of techniques to identify at least three different platelet glycoproteins, GP IIa, IIIa and Ib with A and B determinants, of which GPIIa was the most prominent. The authors apply platelet absorption and acid-eluting purified high-titer IgG anti-A, anti-B serum, various glycoprotein monoclonal antibodies by radioimmunoprecipitation, immunoblotting and monoclonal antibody-immobilized platelet antigen method (MAIPA ), On the platelet A, B blood group determinants in the platelet glycoprotein localization, made an accurate determination.