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选初断乳ＳＤ大鼠，用两个水平蛋白质（酪蛋白１２％和２０％）、淀粉（３０％和６５％）、核黄素（１２μｇ／ｄ和２４μ／ｄ）组成２×２析因实验，研究大鼠核黄素营养状况及需要量与膳食因素的关系，实验期８周。结果表明，核黄素摄入量增加，动物生长、肝脏核黄索浓度和全血谷耽甘肽还原酶活性系数（ＢＧＲＡＣ）均明显改善。增加蛋白质摄入量，核黄素在肝脏中储备增加，但同时ＢＧＲＡＣ上升，提示组织可利用核黄素水平降低。淀粉摄入量增加，肝脏核黄素浓度和ＢＧＲＡＣ均有改善，提示整体核黄素营养水平有所提高。实验显示，核黄素需要量与膳食中蛋白质和淀粉摄入量有关，高蛋白、低淀粉组动物，核黄素需要量相对较高。
Primary weaned Sprague-Dawley (SD) rats were randomly divided into 2 × 2 factors (12 μg / d and 24 μ / d) with two horizontal proteins (12 and 20% casein and 30 and 65% Experimental study of riboflavin nutritional status and the relationship between dietary requirements and the experimental period of 8 weeks. The results showed that the increase of riboflavin intake, animal growth, heparin concentration and whole blood glutathione reductase activity coefficient (BGRAC) were significantly improved. Increase in protein intake, riboflavin in the liver increased reserves, but at the same time BGRAC increased, suggesting that tissue available riboflavin levels. Increased starch intake, liver riboflavin concentration and BGRAC have improved, suggesting that the overall level of riboflavin nutrition has increased. Experiments show that riboflavin requirements and dietary protein and starch intake, high protein, low starch group of animals, riboflavin demand is relatively high.