从钻天杨(Populus nigra Italica)、美洲黑杨与欧洲黑杨杂交种“弗累瓦杨”(P. Fle-vo)和滇杨(P. yunnensis)的发叶枝或休眠枝上切取腋芽,浸泡于乙醇溶液中进行表面消毒,并用火烤一下,再浸于0.2%次氯酸盐溶液中,然后用无菌水冲洗数次。腋芽经取掉外包皮后置于培养基1中(参见表1)。培养条 Axillary buds were excised from the leaf or dormant branches of Populus nigra Italica, Populus nigra and Populus hybrids “P. Fle-vo” and P. yunnensis, soaked in Ethanol solution surface disinfection, and roasted with a bit, and then immersed in 0.2% hypochlorite solution, and then rinsed several times with sterile water. The axillary buds were placed in culture medium 1 after removal of the outer sheath (see Table 1). Cultures