论文部分内容阅读
目的：建立一种确实有效的更实用的小量质粒DNA的提取方法。方法：将一含有目的质粒的大肠杆菌扩增后采用改良后的方法提取质粒DNA，然后采用紫外分光光度计测定含量，并且进行酶切。结果：每毫升原细菌培养物质粒DNA的产量明显增高，且酶切效果极好。结论：改良后的质粒DNA提取法具有速度快、收获量多、纯度高、经济又方便等优点，适合于应用与推广。
OBJECTIVE: To establish a more effective and practical method for the extraction of small amount of plasmid DNA. Methods: A plasmid containing Escherichia coli was amplified after the use of improved methods to extract plasmid DNA, and then use UV spectrophotometer to determine the content, and the digestion. Results: The yield of plasmid DNA per ml of primary bacterial culture was significantly increased and the digestion result was excellent. Conclusion: The improved plasmid DNA extraction method has the advantages of fast speed, high yield, high purity, economy and convenience, which is suitable for the application and popularization.