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目的探讨含碘消毒剂对临床耐药菌株最小杀菌浓度(MBC)。方法以安尔碘为研究对象,采用对倍稀释法,以不同浓度对临床耐药菌株和标准菌株大肠埃希菌(8099)和金黄色葡萄球菌(ATCC 6538)分别作用1、3、5 min,观察杀灭效果。结果安尔碘的有效碘含量为7.81 mg/L,作用1 min可将所有检测菌全部杀灭;有效碘含量为3.90 mg/L,作用5 min可将检测的所有菌株全部杀灭。但作用1 min,革兰阳性菌中有7株临床分离的金黄色葡萄球菌的MBC(7.81 mg/L)高于其标准菌株(3.90 mg/L);作用5 min,革兰阴性菌中有18株菌的MBC(3.90mg/L)高于标准菌株大肠埃希菌(<1.95 mg/L)。结论绝大部分临床多重耐药菌对安尔碘的抗力未超过标准菌株,仍呈现出良好的杀灭效果。
Objective To investigate the minimum bactericidal concentration (MBC) of iodine-containing disinfectants on clinical drug-resistant strains. Methods Arsenic iodine was used as the research object. The double dilution method was used to treat clinical drug-resistant strains and standard strains of Escherichia coli (8099) and Staphylococcus aureus (ATCC 6538) at different concentrations for 1,3,5 min , Observe the killing effect. Results The effective iodine content of arsenic iodine was 7.81 mg / L, and all the tested bacteria could be completely killed after acting for 1 min. The available iodine content was 3.90 mg / L and all the tested strains were completely killed after 5 min. However, the MBC (7.81 mg / L) of seven clinically isolated Staphylococcus aureus isolates in Gram-positive bacteria was higher than that of the standard strain (3.90 mg / L) for 1 min. After 5 min, the gram-negative bacteria The MBC (3.90 mg / L) of 18 strains was higher than that of the standard strain Escherichia coli (<1.95 mg / L). Conclusion Most of the clinical multidrug-resistant bacteria did not surpass the standard strain for Arsenite, and showed good killing effect.