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采用微滴定板和酶联免疫分析仪测定抗坏血酸，比常规光度法简便快速。磷钼蓝法与邻二氮 菲－铁（Ⅲ）法双对数回归方程相关系数分别为 ０． ９９９８和 ０． ９９９９，最低检出限分别为 １．０４ｍｇ／孔和 ０．０２９μｇ／孔，回收率分别为９９．２％和９９．３％，测得维生素Ｃ片的含量分别为标示量的９７．５％和 ９７.３％。邻二氮菲－铁（Ⅲ）法灵敏度高且比常规光度法操作简便，快速灵敏。
Determination of ascorbic acid using microtiter plates and enzyme-linked immunosorbent assay is simpler and faster than conventional spectrophotometry. Phosphomolybdate blue method and phenanthroline - iron (Ⅲ) double logarithmic regression equation correlation coefficients were 0. 9998 and 0. 9999, the lowest detection limits were 1.04mg / well and 0.029μg / well, the recoveries were 99.2% and 99.3%, respectively. The contents of vitamin C tablets were 97.5% And 97.3%. Phenanthroline - iron (Ⅲ) method of high sensitivity and more convenient than conventional photometry, fast and sensitive.