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在生长季节分3次对部分葡萄品种枝条上部、中部和下部叶片及韧皮部,进行双抗体夹心法(DAS-ELISA)、反转录聚合酶链式反应(RT-PCR)和免疫捕捉反转录聚合酶链式反应(IC-RT-PCR)3种方法检测卷叶病毒Ⅲ(GLRaV-3)的比较。结果表明:DAS-ELISA的检出率明显低于RT-PCR和IC-RT-PCR方法。RT-PCR可以检测出RNA提取液为1:10-4的GLRaV-3病毒,但它受到了提取RNA难以及其它物质(如蛋白质、DNA等)干扰的影响,检测难度增加。从检测的灵敏度来看,RT-PCR和IC-RT-PCR比DAS-ELISA灵敏;并且,IC-RT-PCR比其它两种方法较快,整个过程仅需8h。
DAS-ELISA, RT-PCR and immunocapture reverse transcription (RT-PCR) were performed on the upper, middle and lower leaves and phloem of some grape varieties in three growth stages. Polymerase chain reaction (IC-RT-PCR) three kinds of methods to detect leaf curly virus III (GLRaV-3) comparison. The results showed that the detection rate of DAS-ELISA was significantly lower than that of RT-PCR and IC-RT-PCR. RT-PCR can detect GLRaV-3 virus with RNA extract of 1: 10-4, but it is difficult to extract RNA and interfere with other substances (such as protein, DNA, etc.). From the detection sensitivity, RT-PCR and IC-RT-PCR are more sensitive than DAS-ELISA; and, IC-RT-PCR is faster than the other two methods, the whole process only 8h.