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通过喂食含有盐酸四环素的蜂蜜水抑制丽蚜小蜂体内Wolbachia的活性,并研究以烟粉虱为寄主时Wolbachia内共生菌对丽蚜小蜂生殖力、寿命和后代性别的影响。结果表明:抗生素处理后丽蚜小蜂产生雄性后代,雄性后代所占比例与抗生素浓度呈显著正相关(r=0.322,P<0.01)。在1mg/mL抗生素浓度下丽蚜小蜂产生的寄生蛹数(黑蛹数)为68.1±8.9,较对照的49.1±4.5显著增加;而雌成蜂寿命与对照无显著差异(抗生素处理15.56±1.55,对照12.50±0.88),且从第4天开始产生的后代全部为雄性。说明Wolbachia的共生对丽蚜小蜂的生殖力具有一定的负面影响,Wolba-chia调控宿主生殖的强度与其密度有关。
The effects of Wolbachia endosymbionts on the fecundity, longevity and sex of offspring were studied by feeding tetracycline-containing honey water and inhibiting the Wolbachia activity. The results showed that there was a significant positive correlation between male offspring and the concentration of antibiotics (r = 0.322, P <0.01). The number of parasitoid pupal (black pupal) produced by A. grisea at the concentration of 1 mg / mL was 68.1 ± 8.9, which was significantly higher than that of control (49.1 ± 4.5), while there was no significant difference between the female and the control (antibiotic treatment 15.56 ± 1.55, control 12.50 ± 0.88), and offspring generated from day 4 were all male. These results suggest that the symbiosis of Wolbachia has a negative effect on fecundity of L. aphid, and the intensity of Wolba-chia regulation of host reproductive density is related to its density.