论文部分内容阅读
目的 :通过观察抗氧化剂Ebselen的干预效果 ,探讨氧自由基 (OFR)在一氧化氮 (NO)缺乏所致高血压、心血管重构中的作用。方法 :将 2 4只Wistar大鼠随机分为 3组 ,每组 8只 :①C组 :正常对照组 ;②L组 :L NAME 5 0mg·kg-1·d-1;③L +E组 :L NAME 5 0mg·kg-1·d-1加Ebselen 30mg·kg-1·d-1。隔周测动脉收缩压 ;8周末处死大鼠采集标本 ,检测血浆、心尖部心肌匀浆组织中NO含量及心肌超氧阴离子 (O-2 )水平、血管紧张素Ⅱ一型受体 (AT1受体 )表达 ;取心脏作病理形态检测。结果 :①L组血压逐渐升高 :自 1周末起 ,对比C组同期血压差异均有统计学意义 (P <0 .0 5 )。Ebselen干预早期对血压升高无明显影响 ,但在 8周末则明显低于L组 (P<0 .0 5 ) ;②L组心肌组织NO水平明显低于C组 ,L +E组则明显高于L组 ;③L组心肌O-2 、AT1受体表达水平 ,明显高于C组 ,L +E组则明显低于L组 ;④L组及L +E组心脏重量 /体重 (HW /BW )较对照组有显著增高 ;L +E组较L组有下降趋势 ,但P >0 .0 5。左室壁厚度L组及L +E组均较C组明显增高 (P <0 .0 5 ) ,而Ebselen干预后其左室壁厚度则明显变薄 (P <0 .0 5 ) ;心肌小动脉壁厚 /腔径比值的变化 :L组明显较对照组为大 ,L +E组与L组比较则此种比值有所减小 ,但L +E组仍较对照组为高 (P <0
OBJECTIVE: To investigate the effect of OFR on hypertension and cardiovascular remodeling induced by nitric oxide (NO) deficiency by observing the effect of Ebselen. Methods: Twenty-four Wistar rats were randomly divided into 3 groups with 8 rats in each group: ①C group: normal control group; ②L group: L NAME 50 mg · kg-1 · d-1; ③L + E group: L NAME 50 mg · kg -1 · d -1 plus Ebselen 30 mg · kg -1 · d -1. The systolic pressure of the artery was measured every other week. The rats were sacrificed at the end of the 8th week for the detection of NO content and the level of myocardial O-2 in plasma and apical myocardial homogenate. The expression of angiotensin Ⅱ type 1 receptor Body) expression; take the heart for pathological examination. Results: ①L group blood pressure gradually increased: from the end of 1 week, compared with the C group during the same period blood pressure were statistically significant (P <0.05). Ebselen intervention had no effect on blood pressure in early stage, but was significantly lower than that in L group at the end of 8th week (P <0. 05). ② The level of NO in L group was significantly lower than that in C group and L + E group L group; ③ The expression of O-2 and AT1 receptors in L group were significantly higher than those in C group and L + E group was significantly lower than that in L group; ④ The heart weight / body weight (HW / BW) The control group was significantly higher; L + E group than the L group has a downward trend, but P> 0.05. Left ventricular wall thickness in L group and L + E group were significantly higher than those in C group (P <0.05), while left ventricular wall thickness was significantly thinner in Ebselen group (P <0. 05) Arterial wall thickness / diameter ratio changes: L group was significantly larger than the control group, L + E group compared with the L group, this ratio was reduced, but L + E group was still higher than the control group (P < 0