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目的通过对肝细胞癌(HCC)抑癌基因(tumor suppressor genes,TSGs)杂合性缺失(loss of heterozygosity,LOH)谱系特点的分析,进一步了解HCC发生过程中多基因变异的特点。方法采用显微组织切割法从石蜡包埋的组织切片中提取基因组DNA直接测序,对33例信息性HCC进行了6种TSGs(APC、DCC、MCC、OGG 1、p53和RB1)的LOH检测。结果LOH的总体发生率为36.4％,其中p53:80.0％、OGG1:50.0％、APC:41.7％、RB1:20.0％、DCC:0.O％、MCC:0.0％。结论在HCC的发生和发展过程中有多基因变异的参与,其中p53、OGG1和APC基因在HCC的发生中起重要作用,RB1的作用次之,而DCC和MCC基因的作用可能较小。
Objective To further understand the characteristics of multi-gene variation in the process of HCC by analyzing the characteristics of loss of heterozygosity (LOH) lineage in hepatocellular carcinoma (HCC) tumor suppressor genes (TSGs). Methods The genomic DNA was extracted from paraffin-embedded tissue sections by microscopic cutting. LOH of 6 kinds of TSGs (APC, DCC, MCC, OGG 1, p53 and RB1) was detected in 33 cases of HCC. Results The overall incidence of LOH was 36.4%, among which p53 was 80.0%, OGG1: 50.0%, APC: 41.7%, RB1: 20.0%, DCC: 0.O% and MCC: 0.0%. Conclusions There are many genetic variations in the occurrence and development of HCC. P53, OGG1 and APC genes play an important role in the development of HCC, while RB1 plays a second role, while DCC and MCC genes may play a minor role.