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胰岛素依赖型糖尿病的发病与胸腺T细胞亚群分化异常有关,但对其在发病过程中的分化规律和作用机理尚未完全清楚.本实验给予SD大鼠一次性腹腔注射链脲佐菌素(STZ,65mg/kg)以诱导实验性糖尿病,做常规形态学及免疫组织化学染色观察实验性糖尿病状态下大鼠胸腺的形态结构、胸腺上皮细胞角蛋白(CK)表达及胸腺细胞表面CD4、CD8分子表达的变化.结果表明:本实验所诱导的实验性糖尿病大鼠均均出现典型的糖尿病症状,血糖值均在20.25mmol/L以上,远高于糖尿病大鼠的血糖标准值16.0mmol/L,说明本实验所用的建模方法效果较好.糖尿病大鼠胸腺发生病理性萎缩,CK8+的胸腺皮质上皮细胞相互联结构成的网中存在空洞状缺失,胸腺细胞表面的CD4、CD8表达呈区域状分布,主要分布于胸腺皮质部,髓质部较少见阳性反应细胞.结论:STZ诱导的实验性糖尿病大鼠伴有胸腺萎缩、胸腺皮质上皮缺失及胸腺细胞表面CD4、CD8的表
The pathogenesis of insulin-dependent diabetes is related to the abnormal differentiation of thymus T lymphocyte subsets, but its differentiation and mechanism of action are not fully understood.In this experiment, SD rats were given intraperitoneal injection of streptozotocin (STZ , 65mg / kg) to induce experimental diabetes mellitus, routine morphological and immunohistochemical staining was used to observe the morphology and structure of thymus, the expression of cytokeratin (CK) in thymus and the expression of CD4 and CD8 on thymocytes The results showed that all the experimental diabetic rats induced by this experiment showed typical symptoms of diabetes with blood glucose above 20.25mmol / L, much higher than the standard blood glucose value of 16.0mmol / L in diabetic rats, Indicating that the modeling method used in this experiment is better.Pathological atrophy in the thymus of diabetic rats, CK8 + thymus epithelial cells connected to each other to form a network of empty holes, thymus surface CD4, CD8 expression showed a regional distribution , Mainly distributed in the cortex of the thymus, few positive cells in the medulla.Conclusion: STZ-induced experimental diabetic rats with thymus atrophy, thymus Thymic epithelial cell surface expression and deletion CD4, CD8 of