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目的:研究弓状核在左旋四氢巴马汀(l-THP)镇痛效应中的作用,以阐明l-THP的镇痛作用机制。方法:应用辣根过氧化物酶(HRP)逆行追踪术追踪纹状体或伏膈核与PAG之间的纤维联系,HRP逆行追踪结合免疫组化观察投射神经元的性质,神经核团损毁和PAG核内注射药物观察对l-THP镇痛作用的影响。结果:纹状体或伏膈核通过弓状核或缰核间接与PAG联系,弓状核投射至PAG的神经元大部分是β内啡肽神经元。损毁弓状核后,l-THP的镇痛作用消失,而损毁缰核对l-THP的镇痛作用无明显影响。PAG核内注射纳洛酮能剂量依赖性翻转l-THP的镇痛作用。结论:弓状核的β内啡肽神经元在l-THP镇痛作用中起重要作用。
Objective: To study the role of arcuate nucleus in the analgesic effect of l-tetrahydropalmatine (l-THP) to elucidate the mechanism of l-THP analgesia. Methods: HRP retrograde tracing technique was used to track the relationship between the striatum or the nucleus accumbens and PAG. HRP retrograde tracing combined with immunohistochemistry was used to observe the properties of neurons, Effect of PAG injection on the analgesic effect of l-THP. RESULTS: The striatum or phrenic nerve nuclei were indirectly linked to PAG via the arcuate nucleus or the habenular nucleus. The majority of neurons projecting to the PAG from the arcuate nucleus were β-endorphin neurons. After the arcuate nucleus was destroyed, the analgesic effect of l-THP disappeared, while the damage to the habenular nucleus had no significant effect on the analgesic effect of l-THP. Parenteral administration of Naloxone to PAG attenuated the analgesic effect of l-THP in a dose-dependent manner. CONCLUSIONS: Beta-endorphin neurons in the arcuate nucleus play an important role in the analgesic effect of l-THP.