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目的:探讨尼可地尔预处理对大鼠缺血再灌注心肌mt DNA4834 bp缺失突变的影响。方法:体重200 g-300 g,7-9周龄健康雄性Sprague-Dawley(SD)大鼠50只,随机平均分为5组,假手术(Sham)组、心肌缺血再灌注损伤(MIRI)组、缺血预处理(IPC)组、尼可地尔(Nic)组、尼可地尔+5羟基葵酸钠(Nic+5-HD)组。建立心肌缺血再灌注模型后取心肌组织,提取mt DNA,应用巢式PCR法定性检测mt DNA4834 bp片段缺失突变。结果:MIRI组、IPC组、Nic组和Nic+5-HD组突变频率分别为100%、20%、30%和89%。Sham组未检测到mt DNA4834 bp缺失突变。Nic组mt DNA4834 bp片段缺失明显低于MIRI组和Nic+5-HD组(P<0.05),与IPC组比较差异无统计学意义(P>0.05)。结论:尼可地尔预处理可降低大鼠MIRI后mt DNA4834 bp片段缺失突变,改善线粒体能量衰竭。
Objective: To investigate the effect of nicorandil preconditioning on mt DNA 4834 bp deletion in rat myocardium during ischemia-reperfusion. Methods: Fifty healthy male Sprague-Dawley (SD) rats, weighing 200 g-300 g and 7-9 weeks old, were randomly divided into 5 groups: sham operation group, myocardial ischemia reperfusion injury (MIRI) Group, ischemic preconditioning (IPC) group, nicorandil group and nicorandil + 5 sodium nicotinate (Nic + 5-HD) group. Myocardial tissue was obtained after myocardial ischemia-reperfusion model was established, mt DNA was extracted, nested PCR method was used to qualitatively detect mt DNA 4834 bp fragment deletion mutation. Results: The mutation frequencies of MIRI group, IPC group, Nic group and Nic + 5-HD group were 100%, 20%, 30% and 89% respectively. Sham group did not detect mt DNA4834 bp deletion mutation. The deletion of 4848 bp fragment of mt DNA in Nic group was significantly lower than that in MIRI group and Nic + 5-HD group (P <0.05). There was no significant difference between Nic group and IPC group (P> 0.05). CONCLUSION: Nicorandil preconditioning can reduce mt DNA 4834 bp fragment deletion mutation and improve mitochondrial energy failure after MIRI in rats.