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目的 :观察成年大鼠持续性局灶脑缺血后 TGFβ1 蛋白表达与缺血后凋亡的关系。方法 :单侧大脑中动脉近端电凝术建立大鼠持续性局灶脑缺血模型。缺血后 1h,实验组经尾静脉注射氟美松 (5 mg/ kg) ,对照组注射相同容积的生理盐水。缺血后 3h～ 12 0 h取材 ,分别用免疫组织化学 SP法和原位末端标记 TUNEL法标记 TGFβ1 蛋白表达细胞和凋亡细胞。结果 :持续性局灶脑缺血后 TGFβ1 蛋白呈双相性 ,主要分布在梗塞灶周围区域。TGFβ1 的表达可被氟美松抑制 ,同时缺血后神经细胞凋亡也加重。结论 :大鼠持续性局灶脑缺血后梗塞灶周围区域 TGFβ1 蛋白表达可能有减轻缺血后神经细胞凋亡的作用。
OBJECTIVE: To observe the relationship between TGFβ1 protein expression and apoptosis after ischemia in adult rats with persistent focal cerebral ischemia. Methods: The unilateral middle cerebral artery electrocoagulation was used to establish the model of persistent focal cerebral ischemia in rats. One hour after ischemia, the experimental group was injected with trimethoprim (5 mg / kg) through the caudal vein and the control group with the same volume of saline. The cells were harvested from 3h to 120 h after ischemia, and the expressions of TGFβ1 protein and apoptotic cells were detected by immunohistochemical SP method and TUNEL method respectively. Results: TGFβ1 protein was biphasic after focal cerebral ischemia and mainly distributed in the peri-infarct area. TGFβ1 expression can be inhibited by dexamethasone, and apoptosis of neurons also increases after ischemia. CONCLUSION: The TGFβ1 protein expression in the peri-infarct region after focal cerebral ischemia in rats may reduce the apoptosis of ischemic neurons.