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目的 对比研究在体外细胞培养系统中乙型肝炎病毒（ Ｈ Ｂ Ｖ） 在肝、肾细胞中的复制与表达。方法 用克隆 Ｈ Ｂ Ｖ Ｄ Ｎ Ａ（ｐ１７７ 、ｐ３ ．８ Ⅱ、ｐ Ｃ Ｍ Ｖ３ ．９） 分别转染 Ｈｅｐ Ｇ２ 与２９３ 细胞系；ｐ１７７ 与ｐ３ ．８ Ⅱ还转染了原代人胚肾细胞。培养后收集转染细胞培养上清液，用 Ｅ Ｌ Ｉ Ｓ Ａ 法检测 Ｈ Ｂｓ Ａｇ 与 Ｈ Ｂｅ Ａｇ ；提取转染细胞的 Ｄ Ｎ Ａ 与 Ｒ Ｎ Ａ，进行 Ｓｏｕｔｈｅｒｎ 与 Ｎｏｒｔｈｅｒｎ 印迹杂交检测细胞内 Ｈ Ｂ Ｖ Ｄ Ｎ Ａ 与 Ｈ Ｂ Ｖ Ｒ Ｎ Ａ。结果 转染 Ｈｅｐ Ｇ２ 细胞， Ｈ Ｂｓ Ａｇ 与 Ｈ Ｂｅ Ａｇ 均有高水平表达； Ｓｏｕｔｈｅｒｎ 与 Ｎｏｒｔｈｅｒｎ 印迹杂交显示特异的 Ｈ Ｂ Ｖ Ｄ Ｎ Ａ 与 Ｈ Ｂ Ｖ Ｒ Ｎ Ａ 杂交信号。而转染２９３ 细胞后， Ｈ Ｂｓ Ａｇ 与 Ｈ Ｂｅ Ａｇ 仅出现很低水平表达； Ｓｏｕｔｈｅｒｎ 和 Ｎｏｒｔｈｅｒｎ 印迹杂交除ｐ Ｃ Ｍ Ｖ３ ．９ 转染细胞出现明显的 Ｈ Ｂ Ｖ 特异杂交信号外，其他为阴性或微弱阳性。转染原代人胚肾细胞均无 Ｈ Ｂｓ Ａｇ 与 Ｈ Ｂｅ Ａｇ 表达。结论 Ｈ Ｂ Ｖ Ｄ Ｎ Ａ 在肝细胞中可以复制与表达，在肾细胞中仅有低水平的病毒抗原表达而无复制，只?
Objective To compare the replication and expression of hepatitis B virus (H B V) in liver and kidney cells in in vitro cell culture system. Methods The Hep G2 and 293 cell lines were transfected with the cloned H B V D N A (p177, p3 .8 Ⅱ and p C M V3 .9) respectively; p177 and p3. 8 Ⅱ was also transfected with primary human embryonic kidney cells. After culture, the supernatant of the transfected cells was harvested, and H Bs Ag and H Be Ag were detected by E L I S A method. D N A and R N A of the transfected cells were extracted, and Southern and Northern blot hybridization was used to detect intracellular H B V D N A and H B V R N A. Results Hep G2 cells were transfected with Hbs Ag and H Be Ag at high level. Hybridization between Northern blot and Northern blot showed that H B V D N A hybridized with H B V R N A signal. However, the expression of H Bs Ag and H Be Ag showed only a low level after transfected into 293 cells. Southern and Northern blotting except for p C M V3. 9 transfected cells showed obvious H B V specific hybridization signal, the other was negative or weakly positive. None of the transfected primary human embryonic kidney cells expressed H Bs Ag and H Be Ag. Conclusion H B V D N A can be replicated and expressed in hepatocytes. Only low levels of viral antigens are expressed in kidney cells without replication.