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目的 :检测上海“6 .2 5”事故两例中度骨髓放射病病人 8年后血型糖蛋白A(GPA)变异频率和估算物理剂量的相关性 ;同时测定正常人外周血在体外用γ线照射后红细胞GPA的变化。方法 :取正常人和事故患者外周血 ,分离红细胞并固定 ,在和荧光素标记的单抗结合后用流式细胞仪进行GPA分析 ,计算GPA变异频率 ;以两组正常人血样分批体外照射 1,2 ,5,10Gy ,在照射后放置 1,4 ,6d后进行检测。结果 :与正常人相比 ,两事故病例的GPA变异频率较高 ,其中变异频率较高者其估算受照剂量较高 ;体外照射外周血红细胞达 10Gy也未见GPA变化。 结论 :放射病病例变异红细胞频率与估算剂量间成正相关 ;外周血直接受照中度剂量不能导致GPA变化 ,提示GPA突变反映造血干细胞损伤 ,GPA分析法适合放射病远后效应跟踪研究
OBJECTIVE: To detect the correlation between the frequency of GPA mutation and the estimation of physical dose in two cases of moderate myelosuppression in Shanghai “6.55” accidental accident. The peripheral blood of normal people was also measured in vitro by gamma ray Post-erythrocyte GPA changes. Methods: Peripheral blood was taken from normal and accidental patients. Red blood cells were isolated and fixed. After binding to fluorescein-labeled McAb, GPA analysis was performed by flow cytometry to calculate the frequency of GPA mutation. Two groups of normal human blood samples were irradiated in vitro 1, 2, 5, 10Gy, after exposure to 1,4,6 days after the test. Results: Compared with normal subjects, the frequency of GPA mutation was higher in the two incident cases. The higher the frequency of mutation was, the higher the dose of irradiation was and the higher the frequency of mutation was. There was no GPA change in peripheral blood red blood cells reached 10 Gy in vitro. CONCLUSIONS: The frequency of erythrocyte mutation in patients with radiation sickness is positively correlated with the estimated dose. The moderate and intermediate dose of peripheral blood can not lead to the change of GPA, suggesting that the GPA mutation reflects hematopoietic stem cell injury, and the GPA analysis is suitable for follow-up study of long-term effects of radiation sickness