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目的:建立HPLC同时测定灯延颗粒中灯盏乙素、原阿片碱、芹菜素和延胡索乙素含量的方法。方法:采用Zorbax Extend-C18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈(A)-0.3%醋酸铵水溶液(B),梯度洗脱(0~25 min,15%A;25~70 min,15%~65%A;70~75 min,65%~15%A),流速0.8 m L·min-1,柱温30℃,检测波长分别为280 nm(灯盏乙素、原阿片碱、延胡索乙素)、337 nm(芹菜素)。结果:灯盏乙素、原阿片碱、芹菜素和延胡索乙素进样量分别在0.90~8.99,0.73~7.35,0.05~0.54,0.14~1.37μg线性关系良好;平均回收率分别为99.48%,98.17%,97.41%,98.33%,RSD分别为0.8%,1.2%,1.2%,0.5%。结论:该法适用于同时测定灯延颗粒中灯盏乙素、原阿片碱、芹菜素和延胡索乙素的含量。
Objective: To establish a method for simultaneous determination of scutellarin, protopine, apigenin and tetrahydropalmatine in Dengyan granules by HPLC. METHODS: A mobile phase of acetonitrile (A) -0.3% ammonium acetate in water (B) was eluted with a Zorbax Extend-C18 column (4.6 mm × 250 mm, 5 μm) 70 min, 15% -65% A, 70-75 min, 65% -15% A) at a flow rate of 0.8 m L · min-1 at a detection wavelength of 280 nm Base, tetrahydropalmatine), 337 nm (apigenin). RESULTS: The injection volume of scutellarin, protopine, apigenin and tetrahydropalmatine was good at 0.90 ~ 8.99, 0.73 ~ 7.35, 0.05 ~ 0.54 and 0.14 ~ 1.37μg, respectively. The average recoveries were 99.48%, 98.17 %, 97.41% and 98.33%, respectively. The RSDs were 0.8%, 1.2%, 1.2% and 0.5% respectively. Conclusion: This method is suitable for simultaneous determination of scutellarin, protopine, apigenin and tetrahydropalmatine in Dengyan granules.