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目的：探讨ＩＦＮ－和ＴＮＦ－α在乙型病毒性肝炎发病机制中的作用。方法：采用双抗体夹心ＥＬＩＳＡ法对９６例乙型肝炎患者和３０例健康献血员血清中ＩＦＮ－和ＴＮＦ－α进行检测，用ｔ检验进行统计学分析和相关性分析。结果：各型乙型肝炎患者血清ＩＦＮ－和ＴＮＦ－α水平明显高于对照组（Ｐ＜０．０５）；重型肝炎组（ｘＩＦＮ＝２３４．７±１６．５；ｘＴＮＦ＝３５９．０±１７．２ｐｇ／ｍｌ）与急性肝炎组（ｘＩＦＮ＝１７４．９±１２．０；ｘＴＮＦ＝２２０．６±８．９ｐｇ／ｍｌ）比较，前者均明显高于后者，差异显著（Ｐ＜０．０５）；各型乙型肝炎患者ＩＦＮ－和ＴＮＦ－α呈正相关（ｒ＝０．２４，Ｐ＜０．０５）。结论：ＩＦＮ－和ＴＮＦ－α异常增高可能与肝损伤发生有关。
Objective: To investigate the role of IFN-γ and TNF-α in the pathogenesis of hepatitis B virus. Methods: The serum levels of IFN- and TNF-α in 96 hepatitis B patients and 30 healthy donors were detected by double antibody sandwich ELISA. The t test was used for statistical analysis and correlation analysis. Results: Serum levels of IFN- and TNF-α in patients with various types of hepatitis B were significantly higher than those in the control group (P <0.05); severe hepatitis group (xIFN = 234.7 ± 16.5; xTNF = 359.0 ± 17.2pg / ml) and acute hepatitis group (xIFN = 174.9 ± 12.0; xTNF = 220.6 ± 8.9pg / ml), the former was significantly higher than the latter, the difference was significant (P < There was a positive correlation between IFN-|Ã and TNF-|Á in all types of hepatitis B patients (r = 0.24, P <0.05). Conclusion: The abnormal increase of IFN- and TNF-α may be related to the occurrence of liver injury.