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采用双层培养法，不仅成倍提高了小麦花药愈伤组织的诱导率，而且使愈伤组织分化绿苗的能力有所提高，其绿苗分化率比固体培养提高１２．８％。
The double culture method not only doubled the induction rate of wheat anther callus but also enhanced callus differentiation ability of green seedlings. The differentiation rate of green plantlets was 12.8% higher than that of solid culture.