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目的:建立同时测定椭圆叶花锚中4种主要酮类成分的HPLC方法。方法:采用Kromasil C18柱(4.6mm×300mm,5μm),流动相为甲醇-0.01mol.L-1磷酸氢二钾溶液(pH8.0)(80∶20),流速1.0mL.min-1,紫外检测波长269nm。结果:1-羟基-2,3,5-三甲氧基酮、1-羟基-2,3,4,5-四甲氧基酮、1-羟基-2,3,7-三甲氧基酮、1-羟基-2,3,4,7-四甲氧基酮进样量分别在0.0148～1.48μg(r=0.9999)、0.0296～2.96μg(r=0.9998)、0.0124～1.24μg(r=0.9995)、0.0136～1.36μg(r=0.9996)范围内线性关系良好。平均回收率(n=5)分别为101.0%,98.9%,97.9%,99.5%;RSD分别为2.2%,2.8%,1.4%,2.1%。结论:本法简便、快速、准确,重复性好,可作为椭圆叶花锚中的主要酮类成分的理想检测方法。
OBJECTIVE: To establish an HPLC method for the simultaneous determination of four major chrysenone components in flower anthers of Elliotis. Methods: Kromasil C18 column (4.6 mm × 300 mm, 5 μm) was used. The mobile phase was methanol-0.01 mol·L-1 potassium phosphate dibasic solution (pH 8.0) (80:20) UV detection wavelength 269nm. Results: 1-hydroxy-2,3,5-trimethoxysilane, 1-hydroxy-2,3,4,5-tetramethoxysilane, 1-hydroxy-2,3,7-trimethoxy (R = 0.9999), 0.0296 ~ 2.96μg (r = 0.9998), 0.0124 ~ 1.24μg (r = 0.9995), 0.0136 ~ 1.36μg (r = 0.9996) in the range of good linear relationship. The average recoveries (n = 5) were 101.0%, 98.9%, 97.9% and 99.5%, respectively. The RSDs were 2.2%, 2.8%, 1.4% and 2.1%, respectively. Conclusion: The method is simple, rapid, accurate and reproducible. It can be used as an ideal method for the determination of major ketones in the anchovy.