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1目的 构建重组 HIV- 1SF2株跨膜蛋白 gp41基因片段的原核表达克隆并在大肠杆菌中表达。2方法 通过聚合酶链反应 (PCR)扩增目的基因 ,然后用限制性内切酶将其与原核表达载体 p MAL - p2定向连接 ,构建成重组质粒转入大肠杆菌 DH5α菌中。经 IPTG诱导 ,SDS- PAGE电泳分析有无重组蛋白表达。3结果 获得了重组的原核表达质粒及其表达产物。4结论 在 p MAL - p2中构建的 HIV - 1gp41重组质粒可在大肠杆菌中表达。
1 Objective To construct a prokaryotic expression clone of gp41 gene fragment of recombinant HIV-1SF2 transmembrane protein and express it in E. coli. 2 method, the target gene was amplified by polymerase chain reaction (PCR), and then ligated with the prokaryotic expression vector p MAL - p2 using restriction endonuclease to construct the recombinant plasmid. The recombinant plasmid was transformed into E. coli DH5α. Induction by IPTG, SDS-PAGE electrophoresis analysis of recombinant protein expression. 3 results obtained recombinant prokaryotic expression plasmid and its expression product. 4 Conclusions The recombinant HIV - 1 gp41 plasmid constructed in p MAL - p2 can be expressed in E. coli.