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目的 研究胃腔内促性腺激素释放激素类似物 (GnRH A)对大鼠胃和十二指肠生长抑素免疫阳性细胞及血液、胃液中生长抑素含量的影响。 方法 应用免疫组织化学ABC和ELISA方法分别检测大鼠胃和十二指肠生长抑素阳性细胞的密度及血液、胃液中生长抑素的含量。 结果 胃腔注射GnRH类似物后 ,胃壁内单位面积生长抑素阳性细胞数为 2 6 6± 3 893,与对照组 4 8 3± 6 0 19相比具有差异 (P <0 0 1) ,十二指肠单位面积生长抑素阳性细胞数为 5 1 7± 2 2 14 ,与对照组 5 8 5± 4 4 5 4相比具有差异 (P <0 0 1)。血液中生长抑素ELISA检测的A值为 0 15± 0 0 18,与对照组 0 2 7± 0 0 36相比具有差异 (P <0 0 1) ,胃液中A值为 0 32± 0 0 4 9,与对照组 1 0 2 2±0 36 9相比具有差异 (P <0 0 1)。 结论 外分泌的GnRH对大鼠胃和十二指肠中生长抑素的分泌起显著的抑制作用。
Objective To study the effects of GnRH A on gastric somatostatin - immunoreactive cells and somatostatin in blood and gastric juice in rats. Methods Immunohistochemical ABC and ELISA were used to detect the density of somatostatin-positive cells in stomach and duodenum and the contents of somatostatin in blood and gastric juice respectively. Results After intragastric administration of GnRH analogues, the number of somatostatin positive cells per unit area in the stomach wall was 26 ± 3 893, which was significantly different from that of the control group (P <0.01) The number of somatostatin positive cells per unit area of duodenum was 51.7 ± 2214, which was significantly different from that of the control group (P <0.01). The A value of somatostatin ELISA in blood was 0 15 ± 0 0 18, which was significantly different from that of control group 0 2 7 ± 0 0 36 (P 0 01) and that of gastric juice was 0 32 ± 0 0 4 9, which was different from that of control group 1 0 2 2 ± 0 36 9 (P 0 01). Conclusion Exocrine GnRH can significantly inhibit the secretion of somatostatin in the stomach and duodenum of rats.