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目的探讨食管癌患者肿瘤引流淋巴结中淋巴细胞亚群的特点。方法选取2014年4月—2015年3月收治的食管癌患者66例,其中转移29例、未转移37例,对肿瘤引流淋巴结细胞进行无菌分流,检测CD3~+、CD4~+等亚群在总淋巴细胞中占有的比例,并分析Treg细胞与淋巴细胞之间的相互关系,数据采用SPSS17.0软件进行统计学分析,P<0.05为差异有统计学意义。结果食管癌患者肿瘤引流淋巴结中的Treg细胞与T淋巴细胞、CD4~+细胞之间的比例具有一定的相关性(r=-0.341、-0.351,均P<0.05),未转移组T细胞、B细胞比例明显高于转移组患者,CD4~+、CD8~+细胞比例低于转移组,比较差异均有统计学意义(t=9.362、6.520、5.206、4.008、4.017,均P<0.05)。结论食管癌患者肿瘤引流淋巴结中淋巴细胞亚群存在分布紊乱的现象,Treg细胞与淋巴细胞亚群之间有着一定的关系,对于促进淋巴结转移具有较大的影响。
Objective To investigate the characteristics of lymphocyte subsets in the draining lymph nodes of patients with esophageal cancer. Methods Sixty-six patients with esophageal cancer who were treated from April 2014 to March 2015 were selected, including 29 cases of metastasis and 37 cases of non-metastasis. Apoptotic lymph node cells were aseptically shuned to detect CD3 +, CD4 + In the proportion of total lymphocytes, and analyze the correlation between Treg cells and lymphocytes, the data were analyzed by SPSS17.0 software, P <0.05 for the difference was statistically significant. Results The proportion of Treg cells, T lymphocytes and CD4 ~ + cells in esophageal cancer patients had a certain correlation (r = -0.341, -0.351, both P <0.05) The percentage of CD4 ~ + and CD8 ~ + cells in the metastatic group was significantly lower than that in the metastatic group (t = 9.362,6.520,5.206,4.008,4.017, all P <0.05). Conclusion The distribution of lymphocyte subsets in tumor-draining lymph nodes of patients with esophageal cancer is disordered. There is a certain relationship between Treg cells and lymphocyte subsets, which has a greater impact on the promotion of lymph node metastasis.