论文部分内容阅读
目的探讨铀矿尘作用于小鼠RAW 264.7细胞一定时间后羟自由基(.OH)、过氧化氢(H2O2)及丙二醛(MDA)含量的变化。方法用120μg/ml的铀矿尘混悬液作用于小鼠RAW264.7细胞,分别在作用2、4、8、16、24和32 h后,采用噻唑蓝比色法(MTT法)检测RAW264.7细胞存活率;HE染色镜下观察细胞形态学变化;比色法测定细胞上清液中.OH、H2O2及MDA含量的改变。结果铀矿尘作用于RAW264.7细胞2、4、8、16、24、32 h后,可见铀矿尘对RAW264.7细胞的增殖有明显抑制作用,染尘32 h后细胞存活率仅有(28.37±3.3)%;铀矿尘在作用于RAW264.7细胞16、24、32 h后,镜下可见梭型或不规则型细胞、多核细胞明显增加,细胞胞质缺失,胞核外露和染色变浅,细胞数目明显减少;铀矿尘处理的细胞上清液中.OH、H2O2及MDA在染尘后24 h达到峰值,分别为(94.05±4.72)U/ml、(68.83±4.34)mmol/L、(5.67±0.29)nmol/ml,与对照组比较,差异有统计学意义(P<0.01)。结论铀矿尘可明显抑制RAW264.7细胞的增殖作用,导致细胞死亡数量增多。可引起细胞上清液中.OH、H2O2及MDA含量增高,对RAW264.7细胞具有氧化损伤作用。
Objective To investigate the changes of hydroxyl radical (.OH), hydrogen peroxide (H2O2) and malondialdehyde (MDA) in RAW 264.7 cells exposed to uranium dust. Methods RAW264.7 cells were treated with a suspension of uranium dust (120 μg / ml). After 2, 4, 8, 16, 24 and 32 h of treatment, MTT assay was used to detect RAW264.7 cells .7 Cell viability; Morphological changes of cells were observed under HE staining; Changes of .OH, H2O2 and MDA in the cell supernatant were measured by colorimetric method. Results After 2,4,8,16,24,32 h of exposure to uranium mine dust, the uranium mine dust could significantly inhibit the proliferation of RAW264.7 cells. After 32 h of exposure to dust, the cell survival rate was only (28.37 ± 3.3)%, respectively. Uranium mine dust showed spindle or irregular cells after 16, 24 and 32 h treatment on RAW264.7 cells. The number of multinucleated cells increased obviously, The number of cells in the supernatant of uranium dust-treated cells reached the peak at 24 h after dying, respectively (94.05 ± 4.72) U / ml, (68.83 ± 4.34) mmol / L and (5.67 ± 0.29) nmol / ml, respectively. The difference was statistically significant (P <0.01). Conclusion Uranium dust can significantly inhibit the proliferation of RAW264.7 cells, leading to an increase in cell death. Can cause cell supernatant OH, H2O2 and MDA increased, on RAW264.7 cells with oxidative damage.