论文部分内容阅读
目的 探讨血管内皮生长因子(VEGF)-634G/C基因多态性与汉族儿童紫癜性肾炎(HSPN)的关系.方法 应用聚合酶链反应-限制性内切酶片段长度多态性(PCR-RFLP)技术对100例过敏性紫癜(HSP)汉族儿童进行VEGF-634G/C基因型分析,其中包括合并紫癜性肾炎50例(HSPN组),无合并肾炎者50例(单纯HSP组).50例年龄、性别匹配的健康汉族儿童作为对照组.并采用酶联免疫吸附试验(ELISA)检测各组血浆VEGF水平.结果 HSPN组VEGF-634CC基因型(32%)和C等位基因频率(56%)均高于对照组(分别为10%和33%)及单纯HSP组(分别为10%和35%,P<0.01).HSP患儿中,CC基因型者肾炎的发生率比GG基因型者明显增加,差异有显著性意义(76%vs 31%,P<0.01).CC基因型者血浆VEGF水平(180.5±40.7 pg/mL)较CG(145.2±48.3pg/mL)及GG(101.5±26.5 pg/mL)基因型者显著上升,差异有显著性意义(P<0.05).结论 VEGF-634G/C基因多态性与儿童HSPN的发生有关.C等位基因可能是儿童HSPN的易感基因.“,”Objective To study the relationship of -634G/C gene polymorphism of vascular endothelial growth facto (VEGF) with Henoch-Schonlein purpura nephritis (HSPN) in children. Methods One hundred ethnic Han children with HSP, including 50 children with concurrent nephritis (HSPN group) and 50 children without nephritis (HSP without nephritis group), were enrolled. Fifty age-, sex- and ethnics-matched healthy children were used as the control group. VEGF-634G/C genotypes were determined by PCR-RFLP. Plasma VEGF levels were measured using ELISA. Results CC genotype distribution (32%) and C allele frequency (56%) in the HSPN group were significantly higher than those in the control group ( 10% and 35% respectively) and the HSP without nephritis group ( 10% and 33% respectively) (P <0.01 ). The incidence of nephritis in HSP patients with CC genotype increased significantly when compared with those with GG genotype (76% vs31%; P <0.01). Plasma VEGF levels in patients with CC genotype (180.5±40.7 pg/mL) were significantly higher than those in patients with CG (145.2 ±48.3 pg/mL) and GG (101.5 ±26.5 pg/mL) genotypes (P <0.05). Conclusions VEGF-634G/C gene polymorphism may be associated with the development of HSPN. C allele may a susceptible gene of HSPN.