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为探讨NKG2D及其配体RAE-1、H60在单倍体相合(haploidentical)造血干细胞移植介导的移植物抗肿瘤效应中的作用,以皮下接种H22细胞的BABL/c×C57BL/6杂交F1代(CB6F1)小鼠为受鼠,以C57BL/6小鼠的骨髓+脾细胞为供者细胞,分别用抗CD90.2、NK1.1和NKG2D单克隆抗体清除供者T细胞、NK细胞和封闭NKG2D受体,进行单倍体相合细胞移植,观察移植后的抗肿瘤效应,并以半定量RT-PCR法检测移植后小鼠肿瘤组织RAE-1、H60的mRNA表达水平。结果发现:清除供者T细胞、NK细胞和封闭NKG2D的移植均导致移植物抗肿瘤效应的下降,MHC单倍体相合细胞移植后肿瘤细胞RAE-1、H60的mRNA表达水平上升。结论:NKG2D及其配体RAE-1、H60在移植物抗肿瘤效应中的起着重要的作用。
To investigate the role of NKG2D and its ligands RAE-1 and H60 in the anti-tumor effect of graft mediated by haploidentical hematopoietic stem cell transplantation, H22 cells were inoculated subcutaneously with BABL / c × C57BL / 6 hybrid F1 (CB6F1) mice were recipients, and the myeloid + splenocytes of C57BL / 6 mice were donor cells. The anti-CD90.2, NK1.1 and NKG2D monoclonal antibodies were used to remove donor T cells, NK cells and NKG2D receptor was blocked and haploid matched cells were transplanted. The antitumor effect after transplantation was observed. The mRNA expressions of RAE-1 and H60 in the tumor tissue were detected by semi-quantitative RT-PCR. The results showed that the removal of donor T cells, NK cells and blocked NKG2D all resulted in the decrease of the antitumor effect of the grafts. The mRNA expression of RAE-1 and H60 in MHC haploidentical cell line increased after transplantation. Conclusion: NKG2D and its ligands RAE-1 and H60 play an important role in the anti-tumor effect of grafts.