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目的观察 RASSF1A 基因对食管癌细胞 EC9706生长的抑制。方法将包含RASSF1A 基因的质粒转染 RASSF1A 表达缺失的 EC9706细胞,建立稳定转染细胞系,通过 MTT 法检测细胞活性和增殖能力、流式细胞仪检测其对细胞周期的影响、裸鼠移植瘤实验检测转染细胞的体内成瘤特性。结果稳定表达 RASSF1A 的 EC9706细胞较对照组细胞的 RASSF1A 蛋白表达增加;细胞生长速度减慢(P<0.01);细胞周期中 G_1期比例增加,S 期比例减少(G_1期:RASSF1A 组细胞:68.53%±3.34%;空质粒组:54.25%±4.61%;未转染组:53.41%±5.60%。S 期:RASSF1A 组细胞:(23.19±5.04)%;空质粒组:(31.81±2.05)%;未转染组:32.09%±1.99%)(P<0.01);同时裸鼠致瘤能力也被抑制(P<0.05)。结论 RASSF1A 基因的外源性表达能显著抑制食管癌细胞在体内外的生长,提示该基因在食管癌的发生发展中发挥重要作用。
Objective To observe the inhibitory effect of RASSF1A gene on the growth of esophageal carcinoma cell line EC9706. Methods Plasmids containing RASSF1A gene were transfected into EC9706 cells with the absence of RASSF1A expression to establish stable transfected cell lines. The cell viability and proliferation were assayed by MTT assay. The effects of RASSF1A on cell cycle were detected by flow cytometry. The in vivo tumorigenicity of transfected cells was examined. Results The expression of RASSF1A protein was increased in RASSF1A-treated EC9706 cells compared with control cells. The cell growth rate was slowed down (P <0.01). The proportion of G_1 phase was increased and the proportion of S phase was decreased (G_1 phase: 68.53% ± 3.34%; empty plasmid group: 54.25% ± 4.61%; untransfected group: 53.41% ± 5.60% .S phase: cells in RASSF1A group: (23.19 ± 5.04)%; empty plasmid group: (31.81 ± 2.05)%; Untransfected group: 32.09% ± 1.99%) (P <0.01). Meanwhile, the tumorigenicity of nude mice was also inhibited (P <0.05). Conclusion The exogenous expression of RASSF1A gene can significantly inhibit the growth of esophageal cancer cells in vitro and in vivo, suggesting that this gene plays an important role in the development of esophageal cancer.