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目的:研究酸枣仁汤对小鼠自发活动的影响,确定酸枣仁汤的安全有效浓度。方法:在9AM分别连续灌胃给予雄性昆明小鼠60g.kg-1,30g.kg-1,15g.kg-1的酸枣仁汤,2.5mg.kg-1酒石酸唑吡坦溶液和灭菌注射用水7天,用动物行为学视频分析系统记录并分析第1、3、5、7天给药后30min内和9PM30min内自发活动的次数,比较各组小鼠的死亡率,用电子天平称量并比较给药前和10天后的体重,用血球计数仪检测外周血常规并比较红细胞和血红蛋白的计数。结果:各组小鼠生存率均为100%。与空白对照组比较,各给药组在给药前后的体重和停药3天后外周血中红细胞、血红蛋白的数量均无显著性差异,60g.kg-1,30g.kg-1酸枣仁汤组和酒石酸唑吡坦组小鼠在给药第1、3、5、7天9AM给药后30min内自发活动明显减少,差异具有统计学意义(P<0.05,P<0.01),酒石酸唑吡坦组小鼠在第1天9PM检测的自发活动次数明显减少,差异具有统计学意义(P<0.05),60g.kg-1酸枣仁汤组小鼠在停药3天后9AM的自发活动次数明显减少,差异具有统计学意义(P<0.05)。其余各个时间点各给药组小鼠的自发活动次数少于空白对照组,但是差异无统计学意义。结论:60g.kg-1,30g.kg-1的酸枣仁汤可以减少小鼠自发活动次数,并且无明显毒副作用。
Objective: To study the effect of Suanzaoren Decoction on spontaneous activity in mice and determine the safe and effective concentration of Suanzaoren Decoction. Methods: Male SD mice were given ganoderma lucidum spores solution (60g.kg-1, 30g.kg-1, 15g.kg-1), zolpidem tartrate 2.5mg.kg-1 Water for 7 days, using animal behavior video analysis system to record and analyze the number of spontaneous activities within 30min and 9PM30min after the 1st, 3rd, 5th and 7th days of administration, comparing the mortality of mice in each group, weighed by electronic balance The body weight was compared before administration and after 10 days. Peripheral blood routine was measured by hemacytometer and the counts of erythrocytes and hemoglobin were compared. Results: The survival rate of mice in each group was 100%. Compared with the blank control group, there was no significant difference in the weight of red blood cells and hemoglobin in the peripheral blood before and after administration of the three administration groups, (P <0.05, P <0.01), Zolpidem tartrate group and Zolpidem tartrate group (P <0.05, P <0.01) The number of spontaneous activity detected at 9PM on day 1 was significantly decreased (P <0.05). The number of spontaneous activity of 9g at 60g.kg-1 in Suanzaoren Decoction group was significantly decreased after 3 days of withdrawal , The difference was statistically significant (P <0.05). The rest of the time points of the mice in each administration spontaneous activity less than the number of control group, but the difference was not statistically significant. Conclusion: 60g.kg-1, 30g.kg-1 of Suanzaoren Decoction can reduce spontaneous activity in mice, and no obvious side effects.