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以杜氏利什曼原虫(WR352,WRI168c或Edmael)前鞭毛体膜伍用弗氏不完全佐剂免疫小鼠的脾细胞与NS-1瘤细胞融合,获16株单克隆抗体。以亲和纯化的~(125)I标记的兔抗鼠抗体的F(ab′)2对利什曼原虫前鞭毛体膜进行间接放射免疫结合试验。前鞭毛体
Sixteen monoclonal antibodies were obtained by fusing spleen cells of mice immunized with incomplete Freund’s adjuvant to NSC-1 tumor cells with promastigotes of Leishmania donovani (WR352, WRI168c or Edmael). Indirect radioimmunoassay of Leishmania promastigote membranes with F (ab ’) 2 of affinity-purified ~ (125) I labeled rabbit anti-mouse antibody. Promastigotes body