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研究了核黄素的化学发光性质，建立了一种测定核黄素的化学发光新方法，并应用于实际样品中核黄素的测定，与文献相比，本方法无需避光，而且分析体系和样品处理简单；干扰小，牛血清白蛋白、抗坏血酸、天冬氨酸等８种生物有机物质和 Ｃｕ（Ⅱ）、Ｚｎ（Ⅱ）、Ｆｅ（Ⅲ）等１５种常见金属离子的允许量较大；核黄素溶液浓度在０． １０～５０ ｍｇ／Ｌ范围内，与化学发光强度成正比，方法线性范围宽；测定１．０ｍｇ／Ｌ核黄素溶液１２次，求得相对标准偏差为２．９％，方法精密度高；方法的检测限为０．０７５ ｍｇ／Ｌ，灵敏度高。
The chemiluminescence properties of riboflavin were studied. A new chemiluminescence method for the determination of riboflavin was established and applied to the determination of riboflavin in the actual samples. Compared with the literature, this method does not need to be protected from light, and the analytical system and Sample processing is simple; 8 kinds of bio-organic substances such as bovine serum albumin, ascorbic acid and aspartic acid and 15 kinds of common metal ions such as Cu (Ⅱ), Zn (Ⅱ) and Fe (Ⅲ) ; Riboflavin concentration of 0. The linear range of the method was linear in the range of 10 ~ 50 mg / L, which was proportional to the chemiluminescence intensity. The relative standard deviation (RSD) was 2.9% when the 1.0 mg / L riboflavin solution was determined 12 times. The detection limit of this method is 0.075 mg / L with high sensitivity.