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目的:建立大鼠气虚型颈椎病动物模型。方法:选择3月龄雌性SPF级SD大鼠30只,随机分为正常组、颈椎病模型组和气虚型颈椎病模型组,每组10只。颈椎病模型采用动静力失衡性大鼠颈椎间盘退变模型,气虚型颈椎病模型采用动静力失衡性大鼠颈椎间盘退变模型复合疲劳加饥饱失常法制造的气虚模型而制成。通过动物的行为、体征、体质量、环磷酸腺苷(cAMP)、环磷酸鸟苷(cGMP)、乳酸脱氢酶(LDH)来验证气虚证;通过颈椎间盘组织病理学、Ⅱ型和Ⅹ型胶原的免疫组织化学检测及聚集蛋白聚糖(Agc1)、Ⅱ型前胶原基因(Col2 a1)、基质金属蛋白酶-13(MMP-13)和基质金属蛋白酶抑制剂-1(TIMP-1)的基因表达评判椎间盘退变程度,以证实颈椎病模型的成立。结果:与正常组和颈椎病组比较,气虚型组动物出现明显的精神委靡、倦怠、少动、喜卧、毛乱无光泽、眼睑下垂等气虚证的表现,体质量下降;cAMP、cGMP升高,cAMP/cGMP降低(P<0.05,P<0.01),LDH升高(P<0.05);颈椎间盘组织病理学退变更加明显,Ⅱ型胶原蛋白表达减少,Ⅹ型胶原表达增高,Agc1、Col2 a1和TIMP-1基因表达降低,MMP-13表达增高(P<0.05,P<0.01)。结论:通过病-证模型复合的方式可建立大鼠气虚型颈椎病模型,气虚可以加重颈椎间盘的退变。
Objective: To establish a rat model of cervical syndrome with deficiency Qi. Methods: Thirty female Sprague-Dawley (SD) rats of 3 months old were randomly divided into normal group, cervical spondylotic myelopathy group and model group of cervical spondylosis of deficiency type, with 10 rats in each group. Cervical spondylosis model with dynamic and static imbalance of cervical disc degeneration model, deficiency type of cervical spondylosis model using dynamic and static imbalance of cervical disc degeneration model compound fatigue and hunger insufficiency made of qi deficiency model made. Qi deficiency syndrome was confirmed by animal behavior, signs, body weight, cAMP, cGMP, and LDH. By means of cervical disc histopathology, type Ⅱ and type X Immunohistochemical detection of collagen and expression of Agc1, Col2 a1, MMP-13 and TIMP-1 Expressing the degree of degeneration of the disc in order to confirm the establishment of cervical spondylosis model. Results: Compared with the normal group and the cervical spondylosis group, the body weight of the Qi deficiency group was significantly lower than that of the Qi deficiency group (P <0.05); cAMP, cGMP (P <0.05, P <0.01) and LDH increased (P <0.05). The pathological degeneration of cervical disc was more obvious, the expression of type Ⅱ collagen was decreased and the expression of collagen type X was higher. The expressions of Agc1, The expressions of Col2 a1 and TIMP-1 decreased and the expression of MMP-13 increased (P <0.05, P <0.01). Conclusion: The syndrome model of Qi-deficiency of the cervical spondylosis in rats can be established by way of combination of disease-syndrome model. Qi deficiency can aggravate the degeneration of cervical disc.