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Protein kinase C (PKC) is involved in intra-cellular signal transduction in various physiological and pathological processes including substance abuse. In the present study,the role of PKC in morphine-induced rewarding memory was investigated using the conditioned place preference (CPP) model. We found a significant translocation of PKCs from cytosol to membrane component in nucleus accumbens (NAc) of morphine-conditioned rats in a dose-dependent manner. The translocation was reduced gradually with the spontaneous extinction of morphine-induced CPP. Specifically,the protein level of PKCγ in membrane of the NAc was increased in morphine CPP rats,and decreased during the attenuation of morphine-induced CPP,while the protein level of PKCγ in cytosol of the NAc showed an opposite change. Furthermore,the PKC translocation inhibitor γV5-3 impaired the morphine induced CPP when microinjected into the NAc. These findings indicated that PKC,especially the γ isoform,is essential for the acquisition and maintenance of morphine associated reward memory.
Protein kinase C (PKC) is involved in intra-cellular signal transduction in various physiological and pathological processes including substance abuse. In the present study, the role of PKC in morphine-induced rewarding memory was investigated using conditioned condition preference (CPP) model . We found a significant translocation of PKCs from cytosol to membrane component in nucleus accumbens (NAc) of morphine-conditioned rats in a dose-dependent manner. The translocation was reduced gradually with the spontaneous extinction of morphine-induced CPP. level of PKCγ in membrane of the NAc was increased in morphine CPP rats, and decreased during the attenuation of morphine-induced CPP, while the protein level of PKCγ in cytosol of the NAc showed an opposite change. Furthermore, the PKC translocation inhibitor γV5- 3 impaired the morphine induced CPP when microinjected into the NAc. These Results indicates that PKC, especially the γ isoform, is essential for the acqu isition and maintenance of morphine associated reward memory.