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目的探讨垂盆草提取物(SSBE)对重症急性胰腺炎(SAP)模型大鼠急性肺损伤(ALI)及过度炎症反应的影响。方法将42只健康成年SD大鼠随机分成3组:假手术组(C)、胰腺炎模型组(SAP)及SSBE治疗组,每组14只。逆行胰胆管穿刺注射5%牛磺胆酸钠(1 m L/kg)制备SAP肺损伤大鼠模型,SSBE组在此基础上即刻皮下注射SSBE(100 mg/kg),12 h后同等剂量再注射1次,C组及SAP组给予等量生理盐水注射。造模成功后分别于12、24 h取标本并处死大鼠,光镜下观察胰腺及肺脏病理损伤进行评分,检测各组大鼠腹水及血清淀粉酶(AMS)、肺脏组织湿干比重(W/D)、髓过氧化物酶(MPO)活性及炎性因子(IL-1、IL-6、TNF-α)含量。结果与C组比较,SAP组腹水及血清AMS明显升高(P<0.05);肺组织MPO、IL-1、IL-6、TNF-α含量及W/D比值亦明显升高(P<0.05)。与SAP组比较,SSBE组大鼠12、24 h腹水及血清AMS、肺组织MPO、IL-1、IL-6及TNF-α、W/D比值等指标明显降低(P<0.05,P<0.01)。光镜下观察SSBE组大鼠胰腺组织及肺组织病理损害减轻,其损伤程度介于C组和SAP组之间。结论 SSBE对SAP模型大鼠肺损伤有缓解作用,可能通过减轻SAP大鼠的炎症反应而改善其ALI。
Objective To investigate the effects of SSBE on acute lung injury (ALI) and over-inflammation in rats with severe acute pancreatitis (SAP). Methods Forty-two healthy adult SD rats were randomly divided into three groups: sham operation group (C), pancreatitis model group (SAP) and SSBE treatment group, 14 in each group. The rat model of SAP lung injury was established by injecting 5% sodium taurocholate (1 m L / kg) by retrograde cholangiopancreatography. The SSBE group was subcutaneously injected with SSBE (100 mg / kg) Injection once, C group and SAP group were given the same amount of saline injection. The rats were sacrificed at 12 and 24 hours after successful modeling. The pathological changes of the pancreas and lungs were observed under light microscope. The ascites and serum amylase (AMS), lung wet-dry weight (W / D), myeloperoxidase (MPO) and inflammatory cytokines (IL-1, IL-6, TNF-α) Results Compared with group C, the levels of ascites and serum AMS in SAP group were significantly increased (P <0.05), and the levels of MPO, IL-1, IL-6 and TNF- ). The levels of MPO, IL-1, IL-6 and TNF-α and W / D in lung tissue in 12, 24 h ascites and serum AMS in SSBE group were significantly lower than those in SAP group (P <0.05, P <0.01) ). Under light microscope, the pathological damage of pancreas and lung tissue in SSBE group was alleviated, and the degree of damage was between that in C group and SAP group. Conclusion SSBE can relieve lung injury in SAP rats, and may improve ALI by relieving the inflammatory reaction in SAP rats.