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目的:建立土牛膝药材中齐墩果酸-28-O-β-D-吡喃葡萄糖苷的含量测定方法。方法:采用RP-HPLC法,色谱柱为Hibar C18(250 mm×4.6 mm,5μm),流动相:乙腈-0.4%磷酸(34∶66),流速:1.0 mL·min-1,检测波长:203 nm,柱温:35℃。结果:齐墩果酸-28-O-β-D-吡喃葡萄糖苷在0.1426-2.851μg范围内线性关系良好(r=0.9999),平均回收率为102.4%(n=6),RSD%为1.7%。结论:本方法简便、准确、重复性良好,可用于土牛膝中齐墩果酸-28-O-β-D-吡喃葡萄糖苷的含量测定。
Objective: To establish a method for the determination of oleanolic acid-28-O-β-D-glucopyranoside in Achyranthes bidentata. Methods: The RP-HPLC method was used. The chromatographic column was Hibar C18 (250 mm × 4.6 mm, 5 μm). The mobile phase was acetonitrile-0.4% phosphoric acid nm, column temperature: 35 ℃. RESULTS: Oleanolic acid-28-O-β-D-glucopyranoside showed a good linearity (r = 0.9999) in the range of 0.1426-2.851 μg with an average recovery of 102.4% (n = 6) 1.7%. Conclusion: The method is simple, accurate and reproducible. It can be used to determine the content of oleanolic acid-28-O-β-D-glucopyranoside in Achyranthes bidentata.