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目的：研究丹参成分对四氯化碳（CCl4）损伤原代培养大鼠肝细胞的作用。方法：CCl4致体外原代培养大鼠肝细胞损伤模型，观察肝细胞的ALT活性及超微结构。结果：丹参的乙酸乙酯萃取物（EAE）和正丁醇萃取物（nBE）均可使10mmol／LCCl4损伤的原代培养大鼠肝细胞培养液中的ALT活性显著降低。从乙酸乙酯萃取物中分离出A、B、C、D4种组分；在1．0mg／ml时C、D组分有显著护肝作用。含量较多的D组分经小鼠CC14肝损整体模型验证，亦有显著的护肝作用。D组分经鉴定为丹参的酚酸性成分。结论：丹参的酚酸性成分为主要护肝部分。
Objective: To study the effect of Salvia miltiorrhiza on the primary culture of rat hepatocytes injured by carbon tetrachloride (CCl4). METHODS: Rat hepatocyte injury model was induced by CCl4 in vitro and the ALT activity and ultrastructure of hepatocytes were observed. Results: Both ethyl acetate extract (EAE) and n-butanol extract (nBE) of Salvia miltiorrhiza can significantly reduce the ALT activity in primary cultured rat hepatocyte cultures damaged by 10mmol/LCCl4. The components of A, B, C, and D were separated from the ethyl acetate extract; at 1.0 mg/ml, the C and D components had significant hepatoprotective effects. The more abundant D component was verified by the mouse model of hepatic damage in CC14, which also had significant hepatoprotective effects. The D component was identified as the phenolic acidic component of Salvia miltiorrhiza. Conclusion: The phenolic acid component of Salvia miltiorrhiza is the major hepatoprotective component.