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目的：为了探讨中枢神经系统铅中毒的生化机制，测定染铅鼠脑海马区神经细胞的ＰＫＣ活性的改变。方法：用慢性染铅Ｗｉｓｔａｒ大鼠为动物模型，用［γ－２３Ｐ］ＡＴＰ掺入外源性底物的方法测定ＰＫＣ活性。结果：染铅鼠海马神经细胞内胞浆ＰＫＣ活性明显升高（Ｐ＜０．０１），胞膜ＰＫＣ活性亦升高，但无统计学意义。结论：神经细胞胞浆ＰＫＣ对铅有高度敏感性，可能是铅致神经毒性作用的关键调节物。
OBJECTIVE: To investigate the biochemical mechanisms of lead poisoning in central nervous system (CNS), the changes of PKC activity in neurons in hippocampus of lead-exposed rats were determined. Methods: Chronic Wistar rats were used as animal models to measure the activity of PKC by the incorporation of [γ-23P] ATP into exogenous substrate. Results: PKC activity in cytoplasm of hippocampus of lead-exposed rats was significantly increased (P <0.01), PKC activity was also increased, but there was no statistical significance. CONCLUSION: PKC in neurons is highly sensitive to lead and may be a key mediator of lead-induced neurotoxicity.