【摘 要】
：
试验旨在通过脂质体转染法检测pIMAU-EGFP载体在山羊骨骼肌细胞中的表达情况,并探索其最佳的转染条件.在构建的适合携带外源基因的山羊骨骼肌特异表达载体pIMAU-EGFP基础上,
【机 构】
：
内蒙古农业大学动物科学学院,呼和浩特市疾病预防控制中心
论文部分内容阅读
试验旨在通过脂质体转染法检测pIMAU-EGFP载体在山羊骨骼肌细胞中的表达情况,并探索其最佳的转染条件.在构建的适合携带外源基因的山羊骨骼肌特异表达载体pIMAU-EGFP基础上,以绿色荧光蛋白为报告基因,采用脂质体转染法检测了pIMAU-EGFP载体在山羊骨骼肌细胞中的表达,并对转染条件进行了摸索.试验结果表明,成功把测序验证正确的pIMAU-EGFP载体转染到山羊骨骼肌细胞中,筛选的最佳转染条件:脂质体与质粒的最佳比例为1∶2,质粒浓度为3.5 μg/μL,细胞密度不低于80％,以转染24～36 h观察记录转染效率为宜.本试验初步建立了转染山羊骨骼肌细胞的方法,绿色荧光蛋白可作为报告基因优化脂质体转染条件,这将为快速和规模化筛选山羊肉质特性相关基因提供新思路和研究方法.
其他文献
AIM: To determine factors affecting the outcome of patients with cirrhosis undergoing surgery and to compare the capacities of the Child-Turcotte-Pugh (CTP) and
Background The failure of hormone treatment for advanced prostate cancer might be related to aberrant activation of the androgen receptor.We have shown that 125
The canalicular membrane represents the excretory pole of hepatocytes. Bile is an important route of elimina-tion of potentially toxic endo- and xenobiotics (in
Background Tumstatin is a recently developed endogenous vascular endothelial growth inhibitor that can be applied as an anti-angiogenesis and antineoplastic age
Hepatic steatosis affects 20% to 30% of the general adult population in the western world. Currently, the technique of choice for determining hepatic fat deposi
介绍了在体观测肿瘤内注射吲哚氰绿 (ICG) ,一种激光吸收染剂前后X射线衰减的实验结果。实验采用已种植两周的骨髓瘤细胞 (SP2 / 0 ) ,质量为 15～ 2 0g的BALB/c小鼠作为动物模
Background The dialysis disequilibrium syndrome is characterized by neurologic deterioration and cerebral edema which occurs after hemodialysis. The purpose of
Background Colon cancer is a common malignant tumor in the clinic with an incidence rate that is increasing in recent years. The key point for improving the sur
Background In recent years, interventional tumor therapy, involving implantation of intra-cholangial metal stents through percutaneous trans-hepatic punctures,
Crofton weed is an Invasive weed in southwestern China.The activities of several antioxidative enzymes involved in plant protection against oxidative stress wer