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目的:探讨伊诺舒注射液(Ambroxol Hydrochloride Injection,AHI)对高氧诱发新生鼠肺损伤的影响。方法:选用新生Wistar乳鼠120只,随机分为3组(每组n=30),空气对照组、高氧模型组、AHI治疗组。空气对照组新生鼠暴露在室内空气中,其他各组新生鼠暴露在90%O2共14天,实验开始第2天治疗组腹腔内注射AHI(50 mg/kg),空气对照组和高氧模型组腹腔内注射生理盐水,实验开始第3、7及14天每组处死10只动物,取支气管-肺泡灌洗液(bronchoalveolar lavage fluid,BALF)检测丙二醛(MDA)水平。光镜下观察肺组织的病理变化,采用免疫组化染色观察肺组织中结缔组织生长因子(CTGF)和肿瘤坏死因子-α(TNF-α)的表达和Clara细胞的数量。结果:高氧模型组BALF中MDA水平、肺组织中CTGF及TNF-α的表达强度明显高于空气对照组,Clara细胞数明显低于空气对照组,均有显著性差异(均P<0.01);AHI治疗组BALF中MDA水平、肺组织中CTGF及TNF-α的表达强度明显低于模型组,Clara细胞数明显高于模型组,均有显著性差异(均P<0.01)。结论:AHI对新生大鼠高氧肺损伤有保护作用。
Objective: To investigate the effect of Ambroxol Hydrochloride Injection (AHI) on lung injury in neonatal rats induced by hyperoxia. Methods: One hundred and twenty newborn Wistar rats were randomly divided into 3 groups (n = 30 each), air control group, hyperoxia model group and AHI treatment group. The newborn rats of the air control group were exposed to the indoor air. The other newborn rats were exposed to 90% O2 for 14 days. On the second day after the start of the experiment, AHI (50 mg / kg) The rats in each group were sacrificed on the 3rd, 7th and 14th days after the start of the experiment. Ten animals were killed in each group. Bronchoalveolar lavage fluid (BALF) was used to detect the level of malondialdehyde (MDA). The pathological changes of lung tissue were observed under light microscope. The expression of connective tissue growth factor (CTGF) and tumor necrosis factor-α (TNF-α) and the number of Clara cells in lung tissue were observed by immunohistochemical staining. Results: The level of MDA in BALF and the expression of CTGF and TNF-α in lung tissues in hyperoxia model group were significantly higher than those in air control group. The number of Clara cells was significantly lower than that in air control group (all P <0.01) The level of MDA in BALF of AHI treatment group was significantly lower than that of model group (P <0.01). The expression of CTGF and TNF-α in lung tissue of AHI treatment group was significantly lower than that of model group. Conclusion: AHI has a protective effect on hyperoxic lung injury in neonatal rats.