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目的:通过对微生物实验室参加FEPAS比对能力验证计划实施及结果分析,了解国际间微生物检测整体水平,探讨提高能力验证工作有效控制方法和措施。方法:采用传统生理生化鉴定方法和VITEK全自动微生物鉴定系统相结合的办法,充分考虑检测过程中可能影响结果的因素,对目标微生物进行准确的定量或定性。结果:所采用的方法能准确检测样品中的目标菌,8项FEPAS比对能力验证计划均获得满意成绩。结论:FEPAS比对能力验证计划结果反应了各参加实验室微生物检测能力和水平。积极参与FEPAS比对能力验证计划对提高微生物实验室整体检测水平的提升具有重要的促进作用。
OBJECTIVE: To understand the implementation of the proficiency testing program of FEPAS in Microbiology Laboratory and to analyze the results, to understand the overall level of international microbial tests and to explore ways and measures to effectively control capacity proficiency testing. Methods: By combining the traditional physiological and biochemical methods and VITEK automatic microbial identification system, the factors affecting the results in the detection process were fully considered, and the target microorganisms were accurately quantified or characterized. Results: The method adopted could accurately detect the target bacteria in the samples, and satisfactory results were obtained in all eight FEPAS proficiency testing programs. CONCLUSIONS: The FEPAS proficiency test results reflect the ability and level of microbial testing in participating laboratories. Active participation in FEPAS proficiency testing program to improve the overall level of microbial laboratory to enhance the detection has an important role in promoting.