目的检测血清癌胚抗原(CEA)、糖蛋白抗原19-9(CA19-9)、CA125、CA72-4和CA50在结直肠癌(CRC)患者血清表达水平,探讨肿瘤标志物检测在结直肠癌诊断中的意义。方法选择157例结直肠癌患者为CRC组,67例结直肠良性病变患者为良性病变组,以100例健康体检者为对照组。统计分析3组血清5项肿瘤标志物检测结果,并对单项和5项联合检测进行方法学比较。结果 CRC组5项肿瘤标志物表达水平均明显高于良性病变组及对照组;CRC患者Ⅲ期~Ⅳ期除CA72-4外表达水平均明显高于Ⅰ期~Ⅱ期患者,差异有统计学意义(P<0.05);CRC发生肿瘤转移患者各肿瘤标志物均明显高于无转移存在患者;5项联合检测敏感度和准确性均明显高于单项检测,以上差异均有统计学意义(P<0.05)。结论血清5项肿瘤标志物检测对结直肠癌诊断有一定的价值,联合检测可提高灵敏度和准确度。 Objective To detect the serum levels of carcinoembryonic antigen (CEA), glycoprotein antigen 19-9 (CA19-9), CA125, CA72-4 and CA50 in patients with colorectal cancer (CRC) and to explore the correlation between the detection of tumor markers in colorectal cancer The significance of diagnosis. Methods A total of 157 patients with colorectal cancer were selected as CRC group and 67 patients with benign colorectal lesions as benign lesion group. 100 healthy subjects were selected as control group. Statistical analysis of three groups of serum tumor markers in 5 test results, and a single and five joint test methodological comparison. Results The expression levels of five tumor markers in CRC group were significantly higher than those in benign lesion group and control group. The expression levels in stage Ⅲ-Ⅳ of CRC patients except CA72-4 were significantly higher than those in stage Ⅰ-Ⅱ, the difference was statistically significant (P <0.05). The tumor markers of CRC patients with tumor metastasis were significantly higher than those without metastasis. The sensitivity and accuracy of 5 combined tests were significantly higher than those of single test (P <0.05). Conclusion The detection of 5 tumor markers in serum has certain value in the diagnosis of colorectal cancer. Combined detection can improve the sensitivity and accuracy.