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目的 研究胃窦及十二指肠粘膜中促胃液素释放肽(GRP)细胞的免疫细胞化学染色方法,并评价其效果。 方法 PAP法及双PAP法。 结果 GRP细胞主要位于胃腺及十二指肠腺管中下部。双PAP法可大大提高染色阳性强度。 结论 双PAP法是显示胃肠道GRP细胞的较好方法。
Objective To study the immunocytochemical staining of gastrin-releasing peptide (GRP) in the gastric antrum and duodenal mucosa and to evaluate the effect. Methods PAP method and double PAP method. Results GRP cells mainly located in the lower part of the stomach gland and duodenal ducts. Double PAP method can greatly improve the staining positive intensity. Conclusion The double PAP method is a good method to display GRP cells in the gastrointestinal tract.