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用亚洲棉与比克氏棉进行杂交,对杂种F1进行染色体加倍,获得(亚洲棉×比克氏棉)F1双二倍体。(亚洲棉×比克氏棉)F1双二倍体后代中分离出可育和不育两种类型。不育系形态特征与可育系相似,整体性状似父本比克氏棉,多数花为闭花授粉。花粉母细胞减数分裂染色体观察结果表明,不育系与可育系细胞的染色体数目相同,但不育系的单价体较多,染色体构型为2n=52=7.07I+18.13II+2.00III+0.67IV,二价体平均交叉数为1.91;可育系的染色体构型为2n=52=3.09I+23.79II+0.39III+0.04IV,二价体平均交叉数为1.91。另外,不育虽能形成明显的赤道板,但异常的纺缍体使在赤道板上的染色体方向各异,后期I染色体分向两极数目不均等,一些配对染色体成整体运动,而不分向两极。中期II中出现染色体不同步。后期II出现各种畸形孢子群,结果产生各类不育花粉粒。
Hybrids were made with Asian cotton and Becker’s cotton, and the chromosome of hybrid F1 was doubled to obtain F1 double diploid (Asian cotton × Beech cotton). (Asiatic cotton × Beak cotton) F1 double diploid progenies were isolated two types of fertility and infertility. Morphological characteristics of sterile lines and fertile lines similar to the overall performance of the male parent Beech, the majority of flowers for the pollination of flowers. The results of meiosis of pollen mother cells showed that the number of chromosomes in sterile and fertile cells was the same, but the male sterile lines had more monovalent chromosomes with the chromosome configuration of 2n = 52 = 7.07I + 18.13II + 2.00III + 0.67IV, the average cross number of bivalents was 1.91; The chromosome configuration of fertile lines was 2n = 52 = 3.09I + 23.79II + 0.39III + 0.04IV, and the average cross number of bivalents was 1.91. In addition, although infertility can form a significant equatorial plate, but the abnormal spinning body so that the chromosomes in the equatorial plate in different directions, the late I chromosome number two poles uneven, some paired chromosomes into the overall movement, without separation Poles. Chromosomes are not synchronized in metaphase II. Late II appeared a variety of deformed spore groups, resulting in all kinds of sterile pollen grains.