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目的 了解人肺巨细胞癌亚系PLA -80 1C和PLA- 80 1D目前的体内自发转移特性,为进行下一步研究提供具体数据及所需模型。方法 采用肿瘤细胞动物皮下接种的方法,建立肿瘤自发性转移动物模型,实验结束时处死动物行病理学检查,统计成瘤率及转移率。结果 两株细胞的成瘤率均为10 0 %。腋窝皮下接种组PLA- 80 1C和PLA -80 1D的淋巴结转移率分别为0 ,33 .33% ,肺转移率分别为10 0 % ,6 6. 6 7%。左后腿皮下接种PLA 80 1D的裸鼠淋巴结及肺部的转移率分别为16. 6 7% ,4 1 .6 7%。已与前人所验证的结果明显不同。结论 本文分析了这两株细胞的成瘤性和转移性变化的原因,并认为这两株细胞在转移特性上仍存在明显差异,仍具有用于肿瘤转移领域研究的重要价值。
Objective To understand the current in vivo spontaneous metastatic characteristics of human pulmonary giant cell carcinoma sublines PLA-80 1C and PLA-80 1D and to provide specific data and models needed for further study. Methods The animal model of spontaneous metastasis was established by subcutaneous inoculation of tumor cells. The animals were sacrificed at the end of the experiment for histopathological examination. The rates of tumor formation and metastasis were calculated. Results The tumor formation rates of both cells were 100%. The axillary lymph node metastases of axillary subcutaneous group PLA-80 1C and PLA-80 1D were 0, 33.33% and lung metastases were 10 0%, 6 6 6 7% respectively. The metastatic rates of lymph nodes and lungs of nude mice subcutaneously in the left hind leg with PLA 80 1D were 16. 67% and 41.76%, respectively. It is obviously different from the result verified by predecessors. Conclusion The reasons for the changes of tumorigenicity and metastasis of these two cell lines are analyzed. The two cell lines still have significant differences in metastatic characteristics and still have important value in the field of tumor metastasis.